Preliminary Investigation of the Carotenoid Composition of Erythrobacter sp. Strain KJ5 by High-Performance Liquid Chromatography and Mass Spectrometry

Juliadiningtyas, Ayu Dita and Pringgenies, Delianis and Heriyanto, Heriyanto and Salim, Katarina Purnomo and Radjasa, Ocky Karna and Shioi, Yuzo and Limantara, Leenawaty and Brotosudarmo, Tatas Hardo Panintingjati (2018) Preliminary Investigation of the Carotenoid Composition of Erythrobacter sp. Strain KJ5 by High-Performance Liquid Chromatography and Mass Spectrometry. Karna Radjasa, 147 (1). pp. 91-98.

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Abstract

Separation and identification of carotenoids (Cars) from aerobic marine bacterium Erythrobacter sp. strain KJ5 are reported. The cells of Erythrobacter sp. were grown in a Shioi medium at 28.5°C for three days. Among the four solvents tested, the mixture of methanol and acetone (3:7, v/v) was determined as the optimum solvent for Car extraction from the cells by measuring its absorption spectrum. The Cars were separated via reversed-phase high-performance liquid chromatography using a C8 column and identified by a UV-Vis photodiode array detector and an electrospray ionization mass spectrometry. Bacteriochlorophyll a was not detected from the extracts of cells grown under both light and dark conditions. At least 16 peaks of Cars were separated, wherein eleven peaks showed the same absorption spectrum with λmax at 452-453 nm and at 478-480 nm. The other five peaks had an additional absorption peak at 340 nm, which belongs to cis-isomeric form. Two peaks of Cars were identified to be zeaxanthin and β-carotene.

Item Type:	Artikel Jurnal/Prosiding
Uncontrolled Keywords:	β-carotene, carotenoids, Erythrobacter sp., high-performance liquid chromatography, mass spectrometry, zeaxanthin
Subjects:	Q Science > Q Science (General)
Divisions:	Fakultas Teknologi dan Desain > Teknik Sipil
Depositing User:	Admin Repository
Date Deposited:	17 Apr 2020 17:37
Last Modified:	02 Jun 2022 04:20
URI:	http://eprints.upj.ac.id/id/eprint/109

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