Syahputra, M. Rio and Karwur, Ferry F. and Limantara, Leenawaty (2008) Analisis Komposisi dan Kandungan Karotenoid Total dan Vitamin A Fraksi Cair dan Padat Minyak Sawit Kasar (CPO) Menggunakan KCKT Detector PDA. Jurnal Natur Indonesia, 10 (2). pp. 89-97.
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This study was carried out on two phases of Crude Palm Oil (CPO) to determine the total and composition of carotenoid and vitamin A content. Total of carotenoid was analyzed using spectrophotometer UV-Vis, and then the result was calculated by Gross (1991) equation. The vitamin A content was calculated by NAS-NRC equation (1974). The type and composition of both phases of CPO were determined by Choo’s method (1994) by using HPLC with Photo Diode Array (PDA) detector. The sample was prepared in two methods, with and without saponification. The result shows that total carotenoids in liquid and solid phase of CPO are 536 ± 13.2 g/g (liquid), 352 ± 17.7 µg/g (solid) and the vitamin A were 89.4 ± 2.2 RE (liquid), 58.7 ± 3.0 RE (solid), respectively. The carotenoid compositions of both phases of CPO were dominated by - and -carotenes. The result shows that - and -carotenes prepared by saponification method in liquid phase are 29.03% and 60.88%, and without saponification (direct method) are 28.14% and 59.44%. The result for solid phase shows that - and -carotenes by saponification are 25.89% and 60.81%, and without saponification (direct method) are 30.00% and 56.92%. The research also shows the advantages of using HPLC with PDA detector for identification and analysis of type and carotenoid composition.
| Item Type: | Artikel |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Teknologi dan Desain > Teknik Sipil |
| Depositing User: | Admin Repository |
| Date Deposited: | 17 Apr 2020 18:12 |
| Last Modified: | 03 Jun 2022 02:25 |
| URI: | http://eprints.upj.ac.id/id/eprint/116 |
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