Efek Beta Karoten dan Agregasi Klorofil pada Fotostabilitas Klorofil a dalam Pelarut Aseton

da Costa, Junet F. and Karwur, Ferry F. and Limantara, Leenawaty (2009) Efek Beta Karoten dan Agregasi Klorofil pada Fotostabilitas Klorofil a dalam Pelarut Aseton. Jurnal Natur Indonesia, 11 (2). pp. 115-123.

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Abstract

Chlorophyll as photosynthetic pigment has many benefits to human such as antioxidant, antibacterial, color additive, immunity, and photosensitizer. Concerning to these applications, chlorophyll a will react with oxygen and light andreduce its effectiveness. It is then important to understand the stability of chlorophyll a on oxygen and light. Stability of chlorophyll a in the presence of beta-carotene (1:1) in acetone was studied for various waterconcentrations. Each solution was exposed to red light (ë e” 630 nm) for 0.5; 1.0; 2.5; 5.0; 10; 20; 30; 40; 50; and 60minutes and their spectrum were analysed. When water was added (8.33; 16.67; 25; 33.33; 41.67; 50; 58.33; 66.67;75; and 83.33 percent) to the acetone solution of chlorophyll a, the chlorophylls aggregate, and in the presence ofbeta-carotene, the chlorophyll more stable. The water shifts the chlorophyll a spectrum toward red duringillumination with or without the presence of carotene. The formation of oligomeric chlorophyll a aggregate occurswithin 10-20 minutes after the water was applied. This strategy of aggregating the chlorophylls to dissipateexcess energy captured from light does not support the photostability of chlorophyll as much as beta-carotene. Surprisingly, when beta carotene was mixed with 66.7% and 75.0% of water, the chlorophyll a degraded 5.56% and9.71% respectively. In conclusion, the aggregate form of chlorophyll a and the presence of beta-carotene increasephotostability of chlorophyll a in acetone solution.

Item Type: Artikel
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Teknologi dan Desain > Teknik Sipil
Depositing User: Admin Repository
Date Deposited: 18 Apr 2020 19:32
Last Modified: 03 Jun 2022 01:27
URI: http://eprints.upj.ac.id/id/eprint/123

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