Karakterisasi Antosianin Buah Murbei Spesies Morus alba dan Morus cathayana di Indonesia (Anthocyanin Characterization of Morus alba and Morus cathayana in Indonesia)

Sitepu, Rehmadanta and Heryanto, Heryanto and Brotosudarmo, Tatas Hardo Panintingjati and Limantara, Leenawaty (2016) Karakterisasi Antosianin Buah Murbei Spesies Morus alba dan Morus cathayana di Indonesia (Anthocyanin Characterization of Morus alba and Morus cathayana in Indonesia). Karakterisasi Antosianin Buah Murbei Spesies Morus alba dan Morus cathayana di Indonesia, 5 (2). pp. 158-171. ISSN 2338-0950

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Abstract

Mulberry fruit has been known to be rich in antioxidants due to its anthocyanin contents. Unfortunately, the utilization of mulberry fruits as a source of antioxidants and therapy is rare in Indonesia. Mulberry plants are only used for feeding domesticated animal or making tea from its leafs. This study aims to characterize anthocyanin of Morus alba and Morus cathayana. Characterization of anthocyanin exctracts from Morus alba and Morus cathayana were performed using solvent 0.1% hydrochloric acid (HCl) in methanol, and then analized by UV-Vis spectrophotometer and High Performance Liquid Chromatography (HPLC). The results of UV-visible absorption spectra obtained from the crude extract shows that absorption spectra of mulberry extract M. cathayana provide higher absorbance ± 1.3 a.u. compared to absorbance which was obtained from the crude extract of mulberry M. alba are on ± 0.4 a.u. The total anthocyanin obtained from extract of M. cathayana, is 40,39 ± 7,64 mg/g dry weight compared with M. alba which has a value 11,57 ± 3,02 mg/g dry weight. Results of chromatogram of HPLC using XR-ODS column with elution A was 0.1% formic acid in acetonitrile and elution B was 0.1% formic acid in water shows both M. cathayana and M. alba have two dominant pigments of anthocyanin. However, the intensity of chromatograms obtained from M. cathayana is higher than the intensity of chromatograms of M. alba. The intensity of chromatogram of M. cathayana is 50 mAU on retention time 7.86 minutes and 15 mAU on retention time 8.38 minutes. The intensity of chromatogram of M. alba is 10 mAU on retention time 7.35 minutes and 3 mAU on retention time 7.76 minutes. The two dominant anthocyanins are predicted as cyanidin-3-O-glucoside and cyanidin-3-rutinoside

Item Type:	Artikel
Uncontrolled Keywords:	Anthocyanin, Morus alba, Morus cathayana
Subjects:	A General Works > AC Collections. Series. Collected works
Divisions:	Universitas Pembangunan Jaya
Depositing User:	Alexandro Andika
Date Deposited:	13 Apr 2023 06:31
Last Modified:	13 Apr 2023 06:31
URI:	http://eprints.upj.ac.id/id/eprint/4778

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