Riadi, Lieke and Chrisnasari, Ruth and Kristanto, Joshua and Bigravida, Cahaya Caesar and Sanoe, Meyta (2021) How energy efficiency, smart factory, and mass personalization affect companies in Industry 4.0. In: How energy efficiency, smart factory, and mass personalization affect companies in Industry 4.0. INCITE, Surabaya, pp. 1-55. (In Press)
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Abstract
Hydrochloric acid was used to prepare bentonite carrier for xylanase immobilization from Trichoderma reesei as green biocatalyst. The process to produce xylanase from Reutealis trisperma was carried out for 72 hours in batch fermentation at initial pH of 6.0. The xylanase activity was 205.9±0.84 U/mL. It was then purified and analyzed using SDS�PAGE and found to be 26 and 32 kDa. The purification yield was 31.32 %. The bentonite carrier with 140 mesh particle size was pretreated at various HCl concentration. The impurities consist of several ions were successfully replaced by H+ ion as confirmed by FTIR spectra. The carrier was used to immobilize xylanase and the best carrier used in immobilization was bentonite with 2M HCl concentration pretreatment. The best final immobilization was 87.765 % and the leaching was 0.059 %. The biocatalyst can be proposed as green biocatalyst to reduce chlorine used in pulp bleaching.
| Item Type: | Prosiding |
|---|---|
| Uncontrolled Keywords: | xylanase, immobilization, bentonite, green catalyst |
| Subjects: | A General Works > AC Collections. Series. Collected works |
| Divisions: | Universitas Pembangunan Jaya |
| Depositing User: | Alexandro Andika |
| Date Deposited: | 12 Oct 2023 02:10 |
| Last Modified: | 12 Oct 2023 02:10 |
| URI: | http://eprints.upj.ac.id/id/eprint/5470 |
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